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- Title
Regulation of mitochondrial carrier SLC25A13 on breast cancer cell cycle in vitro.
- Authors
Xiao-ping GU; Meng-ping CHEN; A-juan LIANG; Yun-xia LIU; Hai-peng SUN; Ying HUANG
- Abstract
Objective To investigate the role of mitochondrial solute carrier family 25 member 13 (SLC25A13) on breast cancer development. Methods. SLC25A13 mRNA and protein expressions in invasive breast cancer tissues and normal breast tissues were from The Cancer Genome Atlas (TCGA) breast cancer dataset. Survival analysis was conducted online by Kaplan-Meier software. MCF-7 cell line was used for in vitro cell assay. Knockdown of SLC25A13 and sirtuin 2 (SIRT2) were conducted by siRNA transfection. Cell viability was measured with trypan blue exclusion. Cell cycle arrest was determined by flow cytometry. The mRNA expression of SLC25A13 and P27 were detected by quantitative PCR. The protein level of SLC25A13, P27 and SIRT2 were detected by Western blotting. Protein half-life of P27 was assessed by Western blotting after cyclohexinride treatment Results*SLC25AI3 was -regulated in invasive breast cancer tissues. High expression of SLC25A13 correlated with poor overall survival and breast cancer recurrence. SLC25A13 knockdown inhibited MCF-7 cell cycle progression. P27 and SERT2 both accumulated after SLC2SA13 knockdowit P27 accumulation resulted from prolonged protein half-life. Knockdown of SIRT2 restored cell cycle arrest as well as P27 accumulation caused by SLC2SA13 silencing. Conclusion • High expression of SLC25A13 may promote cell cycle progression via SIRT2 in breast cancer development.
- Subjects
PROTEIN expression; BREAST cancer; MESSENGER RNA; CANCER cell culture; SIRTUINS
- Publication
Journal of Shanghai Jiaotong University (Medical Science), 2019, Vol 39, Issue 8, p848
- ISSN
1674-8115
- Publication type
Article
- DOI
10.3969/j.issn.1674-8115.2019.08.007