We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In-Silico Investigation of Cucumber Mosaic Virus (CMV) Resistance Genes and Transcription Factors Involved in CMV Hosts Generated from Chilli-CMV Transcriptome Data.
- Authors
HIREMATH, SHRIDHAR; VINAYKUMAR, H. D.; KOTI, PRASANNA S.; NANDAN, M.; SHANKRAPPA, K. S.; REDDY, C. N. LAKSHMINARAYANA
- Abstract
Cucumber mosaic virus (CMV) severely reduces crop productivity and is considered as a major global plant virus affecting important agricultural and ornamental crops. On infected crops, it causes severe disease symptoms such as mosaic, mosaic mottling, stunting and stem necrosis. Controlling disease propagation has become a serious challenge due to the advent of new strains of CMV, vector biotypes and an expanding host range. The use of resistant cultivars, on the other hand, is the greatest way to tackle this difficulty. In this regard, an attempt was undertaken to explore the previously available chilli-CMV transcriptome data for the presence of resistance (R) genes. Seven R genes were chosen and their physico-chemical characteristics were evaluated. Further, R genes were also extracted from CMV infected hosts using BLAST sequence similarity search tool. The diversity study of R gene proteins revealed that Capsicum annuum R gene proteins clustered closely with Solanum lycopersicum and Nicotiana tabacum R gene proteins and had the highest per cent of amino acid similarity with good query coverage. A venn graph was drawn based on similarity to the proteins database of Oryza sativa, Arabidopsis thaliana, Zea mays and Vitis vinifera to determine the distribution of R gene proteins across diverse crops. Four R gene proteins shared homology with proteins from all of the crops mentioned above. One among the three R gene proteins was found in all crops except Z. mays. The other two R gene proteins were identified in Z. mays, O. sativa and A. thaliana and Vitis vinifera species, respectively. These mined R genes can be used to produce CMV resistance varities. Furthermore, the R proteins can be utilized to study protein-protein interactions.
- Subjects
CUCUMBER mosaic virus; CUCUMOVIRUSES; PLANT viruses; HOST plants; TRANSCRIPTOMES
- Publication
Mysore Journal of Agricultural Sciences, 2021, Vol 55, Issue 4, p355
- ISSN
0047-8539
- Publication type
Article