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- Title
Optimization of glucose oxidase production from marine basidiomycetes by response surface methdology.
- Authors
ZHANG Qingfang; WANG Qianqian; CHI Xuemei; YÜ Shuang; CHEN Ligong; CHI Naiyü; LIU Chunying
- Abstract
Strain 31 originated from sea mud of the Bohai Sea in Dalian. In order to improve GOD enzyme activity of strain 31, based on single factor optimization in the early stage, Plackett-Burman, steepest climbing and Box-Behnken experiments were performed to select the best conditions on enzyme activity. The results showed GOD enzyme activity is significantly improved. The Plackett-Burman experiment determined that the fermentation temperature, the amount of sucrose and inoculum are important factors that affect the enzyme production of strain 31. The steepest climbing experiment and response surface experiment were combined in selecting the optimal culture conditions. The best conditions for the three most significant influencing factors were: the fermentation temperature 26°C, sucrose amount 3.92%, and the inoculum amount 3.58%. According to the actual situation, appropriate adjustments were made to select the optimal conditions for low-temperature GOD enzyme production of strain 31. Under the optimal fermentation conditions, the low-temperature GOD enzyme activity reached to 503.22U/mL. Compared with 435.37 U/mL before the optimization, the GOD enzyme activity increased by 15.58%.
- Subjects
DALIAN (Liaoning Sheng, China); SUCROSE; BASIDIOMYCETES
- Publication
China Food Additives, 2021, Vol 32, Issue 11, p9
- ISSN
1006-2513
- Publication type
Article
- DOI
10.19804/j.issn1006-2513.2021.11.002