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- Title
厚皮甜瓜心部果肉蔗糖含量QTL定位及候选基因分析.
- Authors
杨永; 范蓉; 张学军; 李寐华; 凌悦铭; 张红; 杨文莉; 姜雪; 张永兵; 伊鸿平
- Abstract
【Objective】 This project aims to analyze the content of sucrose in the center flesh of muskmelon by QTL mapping and find out the candidate genes, thus laying a genetic foundation for the genetic improvement of muskmelon with high sucrose content.【Method】 The F2 population was derived from a cross between VZX with high sucrose content as the female parent and HP (high generation inbred lines) with low sucrose content as the male parent. The sucrose content determination was based on high performance liquid chromatography (HPLC). QTL for the sucrose content in the center flesh of muskmelon were detected by bulked segregant analysis (BSA) method and candidate genes were analyzed combined with bioinformatics. 【Results】 The sucrose content in the center flesh of muskmelon was approximately normal distribution in the F2 population, which was consistent with the genetic characteristics of quantitative traits. A total of 32.62 G (average 8.16 G) of valid data were obtained by whole-genome resequencing of the parents and two bulked pools, and a 3.29 Mb interval was located on chromosome 8. A total of 108 genes were annotated. After that, further GO enrichment analysis and KEGG pathway analysis were carried out. A candidate gene EVM0000647 involved in glycolysis and gluconeogenesis in the initial localization interval was obtained by combining transcription data. There were no synonymous mutations in the coding region of EVM0000647 between the high-glucose and low-glucose parents, but there were a lot of different sites in its promoter region, and the expression levels were obviously different between parents. 【Conclusion】 One QTL with 3.29 Mb interval is located on chromosome 8 based on BSA method. EVM0000647gene involved in glycolysis and gluconeogenesis pathway is identified by bioinformatics analysis. There are distinct differences in the expression levels between high-glucose and low-glucose parents, which may play a significant role in the sucrose accumulation in the center flesh of muskmelon.
- Publication
Xinjiang Agricultural Sciences, 2022, Vol 59, Issue 10, p2446
- ISSN
1001-4330
- Publication type
Article
- DOI
10.6048/j.issn.1001-4330.2022.10.013