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- Title
MORPHOLOGICAL AND MOLECULAR CHARACTERISTICS OF FUNGAL ISOLATES CAUSING ONION NECK ROT DISEASE.
- Authors
Kareem, T. A.; Hassan, Aalaa K.; Naemah, Rabab A.; Alsamir, Muhammed
- Abstract
Several Botrytis spp. cause neck rot disease on onion bulbs in the field and storage warehouse in Iraq. This pathogen was isolated from onion bulbs showing symptoms of neck rot disease in different areas of Baghdad. A total of 62 Botrytis spp. isolates were obtained from infected onion bulbs. Pathogenicity of the isolates was investigated on onion and radish seeds in the laboratory. Isolates B4 and B11 were virulent, whilst isolates B15, B10, B9, B7, B6, B2, B20, B19 and B23 were strongly virulent. Results showed that 11 isolates of Botrytis spp. were found in infected Texas Early Grano onion bulbs after incubation at 25 °C for 7 days. Disease incidence rate ranged from 22.2% to 88.9% in the tested isolates. Isolates B10, B6 and B11 had the highest disease incidence rates of 66.7%, 66.7% and 88.9%, respectively and achieved 4.150, 3.547 and 4.360 cm2 lesion areas, respectively on onion bulbs. Morphological characterization revealed a similarity between the selected Botrytis isolates and Botrytis spp.; in specific, B6, B10 and B11 were similar to B. byssoidea; B7 and B2 to B. allii; B9, B15 and B19 to B. squamosa and B4, B2 and B20 to B. porri. Isolates B6, B10 and B11 were molecularly identified based on the specific primer (BA2f/BA1r) by using polymerase chain reaction amplification. A neighbour-joining phylogenetic test was conducted on three isolates under B. byssoidea with seven Botrytis spp. sequences obtained from the NCBI. Results showed that the isolates were grouped with B. byssoidea and closely related to B. aclada. All sequences of B. byssoidea were deposited in GenBank (accession nos. KX181865, KX181864 and KX181863). To the best of our knowledge, this study was the first to record and report on B. byssoidea causing neck rot disease on onion bulbs in Iraq.
- Subjects
BOTRYTIS; ONION diseases &; pests; WAREHOUSES; MICROBIAL virulence; POLYMERASE chain reaction
- Publication
Biochemical & Cellular Archives, 2020, Vol 20, Issue 1, p403
- ISSN
0972-5075
- Publication type
Article
- DOI
10.35124/bca.2020.20.1.403