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- Title
AMELIORATIVE POTENTIAL OF NANOPARTICLES AGAINST 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE (NNK) INDUCED TOXICITY.
- Authors
Jahan, Roshan; Raza, Sana; Dhasmana, Anupam; Lohani, Mohtashim; Mir, Snober S.
- Abstract
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a lung specific carcinogen found in tobacco. Detoxification pathways in the body help to eliminate NNK safely from the body, however, it may get bioactivated and result in the formation of potentially carcinogenic DNA adducts, that may have some toxic manifestations like lung cancer. Some of the nanoparticles have recently been used in scavenging toxicants from contaminated soil and water. We investigated the protective effect of Single walled carbon nanotubes (SWCNTs) and Multi walled carbon nanotubes (MWCNTs) against NNK-induced toxicity in A549 cells. A549 cells when co-exposed to NNK (25 µM, cytotoxic dose and 10 µM sub-cytotoxic dose) along with 0.1 µg/ml, 0.5µg/ml and 1 µg/ml of SWCNTs and MWCNTs, showed significant reduction in the toxic effects of NNK, as measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) Assay, Reactive oxygen species (ROS) Assay and Micronucleus (MN) Assay. Further, in silico studies were performed to explore the mechanism underlying the protective effect of SWCNTs and MWCNTs on carcinogen induced toxicity. Our data revealed that SWCNTs and MWCNTs showed high binding affinity to NNK (-17.16 kcal/mol and - 15.56 kcal/mol, respectively) compared to the binding affinity of NNK with the cellular nicotinic acetylcholine receptor (α7nAChR) (-5.47 kcal/mol) indicating preferential binding of NNK to SWCNTs and MWCNTs. Therefore, our results suggest that the CNTs provide a shield between NNK and the receptors thereby reducing the chances of NNK binding to α7nAChR receptors subsequently resulting into protection against the harmful effects of carcinogens.
- Subjects
SINGLE walled carbon nanotubes; PYRIDYL compounds; LUNG cancer; CARCINOGENS; TETRAZOLIUM compounds
- Publication
Biochemical & Cellular Archives, 2018, Vol 18, Issue 1, p489
- ISSN
0972-5075
- Publication type
Article