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- Title
Authentication of Suzhou Yangcheng Lake Hairy Crab Based on Protein Fingerprint.
- Authors
ZHANG Yahan; ZHANG Youyong; YUAN Dan; XU Min; CAO Yezhong
- Abstract
Crab chelate muscle tissue protein is rich in species and appears in the mass spectrum with different intensity peaks. Crab chelate muscle tissue protein is extracted, and the characteristic index of crab chelate muscle tissue protein spectrum was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology, and the characteristic index is found to achieve the qualitative and quantitative detection of crab chelate muscle tissue protein. In this study, protein molecular fingerprint databases of different categories of crabs were established by analyzing the histoprotein MALDI-TOF MS technology, and the database was deeply analyzed with machine deep learning algorithm to establish the homology analysis method of Yangcheng Lake hairy crabs. The results showed that the protein profiles of hairy crabs in different growing environments showed different expressions. There was an obvious difference when m/z (mass-charge ratio, m/z) was less than 10 000, the scanning results showed that the low m/z region (<10 000) had higher peak intensity and higher sub-marking rate, this region could be used as the mass spectrum resolution region to distinguish Yangcheng Lake hairy crabs from other lake crabs. Above results showed MALDI-TOF MS technique could be used to analyze the different protein expressions of hairy crabs from different places, which could play a role in identifying hairy crab from different places.
- Subjects
MATRIX-assisted laser desorption-ionization; HUMAN fingerprints; PEPTIDE mass fingerprinting; TIME-of-flight mass spectrometry; MACHINE learning; CRABS; DNA fingerprinting
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2023, Vol 25, Issue 11, p80
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2023.0115