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- Title
Antiviral Efficacy Against Hepatitis C NS3/4A Virus Protease by Bioactive Metabolite from Malaysian Fungal Isolate.
- Authors
Sandanamsamy, Sylvia; Ahmad, Rosma; Shin, Lai Ngit; Osman, Hasnah; Fatinathan, Sharon; Perumal, Vikneswari; Balan, Tavamani; Razali, Sharipah
- Abstract
Introduction: Hepatitis C virus (HCV) affects an estimated 150 million people, causing chronic liver disorders. In the absence of a vaccine, standard regimen for patients involves the use of Peg-IFNα in combination with ribavirin or ribavirin in conjunction with boceprevir or telaprevir. However, adverse side effects have occurred in nearly half of the individuals who receive these medications. Crude extracts from diverse Malaysian fungal isolates were evaluated for antiviral activity against HCV NS3/4A protease in the quest for a more effective antiviral medication. Methods: 30 Malaysian fungal isolates were subjected to fermentation in 3 different media. 90 crude fungal fractions were tested for HCV NS3/4A protease activity inhibition. Most potent crude fraction was further fractioned, and subfractions were further tested using HCV NS3/4A protease activity inhibition. Cytotoxicity and GCMS analysis were also performed. Results: HCV protease assay results demonstrated that three methanolic and five ethyl acetate extract samples inhibited HCV protease by more than 95%, which was comparable to quercetin (positive control). An ethyl acetate extract from inhibited HCV NS3/4A protease in a dose-dependent manner with an IC50 value of 64.63g/mL, while cytotoxicity action on Vero cells had a CC50 of 2500 g/mL. The active subfraction contained significant chemicals such as hexadecenoic acid, octadecenoic acid, and adipic acid, according to GCMS analysis. Conclusion: Protease assay yielded excellent results for the fermentative sub-fractions, which can be used to advance the data to a cellbased assay. This study also serves as a framework for testing antiviral activity against different Flaviviridae viruses.
- Subjects
HEPATITIS C virus; OLEIC acid; ADIPIC acid; ETHYL acetate; FUNGAL viruses; CYTOTOXINS; RIBAVIRIN
- Publication
Malaysian Journal of Medicine & Health Sciences, 2023, Vol 19, p45
- ISSN
1675-8544
- Publication type
Abstract