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- Title
Effects of Willing Powder Mediating Notch Pathway on Mice with Nephrotic Syndrome.
- Authors
Luotong JING; Yihan LI; Honglanxi LI; Wenyan ZHANG; Lin QIN; Ning LIANG
- Abstract
[Objectives] This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome (NS) based on Notch pathway. [ Methods ] Sixty KM mice were randomly divided into normal group, model group, prednisone acetate positive group, high-dose Wuling Powder group, medium-dose Wuling Power group and low-dose Wuling Power group, with 10 mice in each group. Three days after prophylactic administration, a comprehensive nephropathy model was prepared by injecting 1 mg/ml doxorubicin hydrochloride solution (7.5 mg/kg) into the tail vein. After successful modeling, prednisone acetate and Wuling SAN were given high, medium and low doses for intervention for 28 d, respectively. After that, urinary protein and creatinine contents of mice in each group were detected, and pathological damage of renal tissue was observed by HE and Masson staining. The mRNA levels of Notchl, Jaggedl and Hesl in mouse kidney tissues were detected by RT-PCR, and the expression levels of Notchl, Jaggedl and Hesl proteins were detected by Western blot. [Results] Wuling Powder could effectively reduce the contents of urine protein (P <0. 01) and Scr (P <0. 01) in NS mice, and alleviate the pathological injury of kidney. Compared with the model group, the prednisone acetate group and various Wuling Powder groups could down-regulate the expressions of Notchl, Jaggedl and Hesl mRNA in the kidney tissue of mice (P<0. 01), and the expression of Notchl protein in the renal tissue of mice decreased (P<0.01). The contents of Hesl in the prednisone acetate group and the high- and medium-dose Wuling Powder groups significantly decreased (P <0. 05). [ Conclusions] Wuling Powder could protect the kidneys in mice with NS through Notch pathway.
- Subjects
NEPHROTIC syndrome; POWDERS; MICE; GENE expression; WESTERN immunoblotting; DOXORUBICIN; KIDNEYS
- Publication
Agricultural Biotechnology (2164-4993), 2023, Vol 12, Issue 6, p25
- ISSN
2164-4993
- Publication type
Article
- DOI
10.19759/j.cnki.2164-4993.2023.06.005