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- Title
Construction of PEG-mediated Genetic Transformation and Gene Knockout System in Fusarium oxysporum f. sp. cubense Tropic Race 4.
- Authors
Lei ZHANG; Yan GUO; Yunyue WANG; Weihua TANG
- Abstract
Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4 (Foc TR4) , is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana industry. In order to reveal the infection process and pathogenesis of Foc TR4, the young mycelia 25 mg/ml driselase, 0.4 mg/ml chitinase, 15 mg/ml lysing enzyme and 1.2 mol/L potassium chloride. The resulted protoplasts of 2 ×107 cells/ml were used to test the efficiency of transformation mediated by polyethylene glycol, and up to 9 transformants per microgram of DNA were obtained. AmCvan, RFP and YFP genes were stably transferred into the WY-Foc TR4, separately, using the protoplast transformation system. The gene FoOCH1 encoding α-1,6-mannosyltransferase in the WT-Foc TR4 was knocked out using the split-marker recombination technology. The genetic transformation and gene kmx-kout system in this pathogen lays a foundation for the study of functional genomics and plant-pathogen interactions.
- Subjects
FUSARIUM oxysporum; GENE knockout; FUSARIUM wilt of banana; GENETIC transformation; PLANT genetic transformation; PLANT-pathogen relationships; FUNCTIONAL genomics; POLYETHYLENE glycol
- Publication
Agricultural Biotechnology (2164-4993), 2020, Vol 9, Issue 1, p15
- ISSN
2164-4993
- Publication type
Article