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- Title
Optimization of Protective Agent Formulation for Soybean Rhizobium Strain HW-05 by Response Surface Methodology.
- Authors
Tao ZHEN; Wei CHEN; Changqing SHA; Xiancheng ZHANG; Xinjie LIU; Deshui YU
- Abstract
[Objective] This study aimed to investigate the protective effects of proteins, salts, sugars and trace elements on soybean rhizobium strain HW-05 by response surface methodology. [Method] Different types, combinations, ratios and concentrations of protective agents were designed. Under simulated conditions, the optima protective agent formulation was screened for improving the survival rate a d survival time of soybean rhizobium strain HW-05. The optimal combination of significant factors was determined by Box-Behnken central composite design. [Result] Three significant factors afecting effective number of viable cells were screened, including peptone, xanthan gum and NaCl. The final concentration of each compound was optimized : 0. 13% peptone, 0. 011% xanthan gum, 0. 30% NaCl. [Conclusion] After addition of protective agent and preservation at room temperature for six months, effective number of viable cehs of soybean rhizobium strain HW-05 reached 3.185 X 10s CFU/ml. The survival rate of HW-05 ceHs was improved by more than 25% compared with the control group (2.458 X 10s CFU/ml).
- Subjects
SOYBEAN; CELL survival; RHIZOBIUM; RESPONSE surfaces (Statistics); MATHEMATICAL optimization
- Publication
Agricultural Biotechnology (2164-4993), 2016, Vol 5, Issue 4, p34
- ISSN
2164-4993
- Publication type
Article