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- Title
Rapid Molecular Identification of Tribolium destructor.
- Authors
ZHANG, Chengwei; XU, Liuping; LU, Miao; LIANG, Xiaosong; LI, Jian
- Abstract
In this study, a rapid molecular identification method of Tribolium, destructor was established with PCR and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technology. According to the results, (1) with PCR method, specific primers were designed based on COI gene of T. destructor for PCR amplification, and electrophoresis detection confirmed that PCR method could be used to rapidly and accurately identify T. destructor; (2) with PCR-RFLP method, two pairs of degenerate primers were used to amplify COI gene of Tribolium species, PCR products were digested with Hindlll and detected by electrophoresis, results indicated that PCR-RFLP method could also be used for rapid identification of T. destructor in quarantine practice.
- Subjects
TRIBOLIUM; TENEBRIONIDAE; POLYMERASE chain reaction; POLYMERIZATION; GENETIC polymorphisms; ELECTROPHORESIS; BIOTECHNOLOGY
- Publication
Agricultural Biotechnology (2164-4993), 2014, Vol 3, Issue 4, p49
- ISSN
2164-4993
- Publication type
Article