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- Title
Yeast Whi2 and Psr1-phosphatase form a complex and regulate STRE-mediated gene expression.
- Authors
Kaida, Daisuke; Yashiroda, Hideki; Toh-e, Akio; Kikuchi, Yoshiko
- Abstract
Abstract Background: In response to various stressful situations, including diauxic conditions, the Msn2 and Msn4 transcription factors induce STRE-mediated gene expression of many stress responsive genes in Saccharomyces cerevisiae . This is called the general stress response. The whi2 cells in the stationary phase are smaller than wild-type cells. Results: Here we demonstrate that STRE-mediated gene expression in whi2 cells is reduced to half of that in the wild-type cells under various stress conditions. It is also delayed for several hours when the mutant cells enter the stationary phase. Using the two-hybrid system, we isolated a WHI2 -interacting gene, PSR1 , which is one of the redundant genes encoding plasma membrane phosphatases. whi2 and psr1 psr2 mutants had similar phenotypes, including reduced STRE-mediated gene expression, higher sensitivity to sodium ions and heat shock, and hyper-phosphorylation of Msn2. The phosphatase activity of Psr1 was necessary for the full activation of STRE-mediated gene expression. Furthermore, both Psr1 and Msn2 were co-immunoprecipitated with Whi2. Conclusions: Thus, Whi2 and its binding partner, Psr1-phosphatase, are required for a full activation of the general stress response, possibly through the dephosphorylation of Msn2. These results may explain why stationary phase whi2 cells are small.
- Subjects
PHYSIOLOGICAL stress; GENE expression; TRANSCRIPTION factors; SACCHAROMYCES cerevisiae
- Publication
Genes to Cells, 2002, Vol 7, Issue 6, p543
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1046/j.1365-2443.2002.00538.x