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- Title
Cyclic di- GMP inhibits Vibrio cholerae motility by repressing induction of transcription and inducing extracellular polysaccharide production.
- Authors
Srivastava, Disha; Hsieh, Meng‐Lun; Khataokar, Atul; Neiditch, Matthew B.; Waters, Christopher M.
- Abstract
Cyclic di- GMP (c-di- GMP) controls the transition between sessility and motility in many bacterial species. This regulation is achieved by a variety of mechanisms including alteration of transcription initiation and inhibition of flagellar function. How c-di- GMP inhibits the motility of Vibrio cholerae has not been determined . FlrA, a homologue of the c-di- GMP binding Pseudomonas aeruginosa motility regulator FleQ, is the master regulator of the V. cholerae flagellar biosynthesis regulon. Here we show that binding of c-di- GMP to FlrA abrogates binding of FlrA to the promoter of the flrBC operon, deactivating expression of the flagellar biosynthesis regulon. FlrA does not regulate expression of extracellular Vibrio polysaccharide ( VPS) synthesis genes. Mutation of the FlrA amino acids R135 and R176 to histidine abrogates binding of c-di- GMP to FlrA, rendering FlrA active in the presence of high levels of c-di- GMP. Surprisingly, c-di- GMP still inhibited the motility of V. cholerae only expressing the c-di- GMP blind FlrA( R176 H) mutant. We determined that this flagellar transcription-independent inhibition is due to activation of VPS production by c-di- GMP. Therefore, c-di- GMP prevents motility of V. cholerae by two distinct but functionally redundant mechanisms.
- Subjects
GUANYLIC acid; VIBRIO cholerae; GENETIC transcription; POLYSACCHARIDES; MOTILITY of microorganisms; BIOSYNTHESIS; GENETIC regulation
- Publication
Molecular Microbiology, 2013, Vol 90, Issue 6, p1262
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.12432