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- Title
Repeatability and reproducibility of microscopic examination of adhesive tape strip cytology slides for the quantification of Malassezia spp. in canine skin.
- Authors
Tapes, Dimitrios; Skampardonis, Vassilis; Chatzis, Manolis K.; Apostolidis, Kosmas; Athanasiou, Labrini V.; Kasabalis, Dimitris; Kokkinaki, Kassiopi Christina G.; Katsogiannou, Eleni G.; Petanides, Theodoros; Leontides, Leonidas; Saridomichelakis, Manolis N.
- Abstract
Background: The optimal microscopic magnification and number of optical fields of adhesive tape strip cytological slides that should be examined when searching for Malassezia yeasts on canine skin are unknown. Objectives: To determine the optimal magnification and the minimum number of optical fields that should be examined to maximise intraobserver repeatability and interobserver reproducibility. Materials and Methods: Seven experienced examiners counted, twice, the number of yeasts in 10, 20, 30, 40 and 50 optical fields of 40 slides at ×400 and ×1000 magnification. Results: The number of yeasts per unit surface area was significantly higher at ×1000 compared to ×400 magnification. Repeatability and reproducibility for counting the yeasts was very poor. Conclusions and clinical relevance: Adhesive tape strip cytological slides should be examined microscopically for Malassezia spp. at ×1000 magnification. The repeatability of this examination for counting the yeasts is poor.
- Subjects
ADHESIVE tape; MALASSEZIA; STATISTICAL reliability; CYTOLOGY; SURFACE area
- Publication
Veterinary Dermatology, 2022, Vol 33, Issue 4, p305
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.13076