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- Title
DIRECT SOMATIC EMBRYOGENESIS OF THE ENDEMIC TAXON Papaver alpinum L. SSP. Corona-sancti-stefani (ZAPAL.) BORZA FOR CONSERVATIVE PURPOSE.
- Authors
Rodica, CATANĂ; Irina, HOLOBIUC
- Abstract
A protocol for regeneration through direct somatic embryogenesis in the endemic taxon Papaver alpinum L. ssp. coronasancti- stefani (Zapal.) Borza was developed. Being both a rare and endemic plant species, this species requires a rescue action using tissue cultures. The somatic embryogenesis was induced from leaf and root explants cultured on MS medium supplemented with different concentrations of cytokinins (BA, kinetin), auxins (2.4-D and NAA) and supplementary additives (mannitol and sucrose). After 90 days of culture at 25ºC, clusters of somatic embryos were obtained. Somatic embryos were developed on all tested media, but the highest number (35 ± 2.43) was recorded on Murashige & Skoog medium supplemented with BA 1 mg/l, NAA 0.2 mg/l and kinetin 1 mg/l. The somatic embryos were converted into plants by transferring on the same medium variant (added with plant growth factors or mannitol) at 10°C. This is the first report of direct somatic embryogenesis in the case of P. corona-sancti-stefani.
- Subjects
CARPATHIAN Mountains; SOMATIC embryogenesis; ENDEMIC plants; REGENERATION (Botany); PLANT germplasm; CYTOKININS; PAPAVER
- Publication
Oltenia, Studii si Comunicari Seria Stiintele Naturii, 2015, Vol 31, Issue 2, p47
- ISSN
1454-6914
- Publication type
Article