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- Title
Revealing proteins associated with symbiotic germination of <italic>Gastrodia elata</italic> by proteomic analysis.
- Authors
Zeng, Xu; Li, Yuanyuan; Ling, Hong; Chen, Juan; Guo, Shunxing
- Abstract
Background: <italic>Gastrodia elata</italic>, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of <italic>G. elata</italic> requires proper fungal association, because of the absence of endosperm. To germinate successfully, <italic>G. elata</italic> obtains nutrition from mycorrhizal fungi such as <italic>Mycena</italic>. However, <italic>Mycena</italic> is not able to supply nutrition for the further development and enlargement of protocorms into tubers, flowering and fruit setting of <italic>G. elata</italic>. To date, current genomic studies on this topic are limited. Here we used the proteomic approach to explore changes in <italic>G. elata</italic> at different stages of symbiotic germination.Results: Using mass spectrometry, 3787 unique proteins were identified, of which 599 were classified as differentially accumulated proteins. Most of these differentially accumulated proteins were putatively involved in energy metabolism, plant defense, molecular signaling, and secondary metabolism. Among them, the defense genes (e.g., pathogenesis-/wound-related proteins, peroxidases, and serine/threonine-protein kinase) were highly expressed in late-stage protocorms, suggesting that fungal colonization triggered the significant defense responses of <italic>G. elata</italic>.Conclusions: The present study indicated the metabolic change and defensive reaction could disrupt the balance between <italic>Mycena</italic> and <italic>G. elata</italic> during mycorrhizal symbiotic germination.
- Subjects
GERMINATION; GASTRODIA elata; PLANT proteomics; ENDOSPERM; MYCORRHIZAL fungi
- Publication
Botanical Studies, 2018, Vol 59, Issue 1, p0
- ISSN
1817-406X
- Publication type
Article
- DOI
10.1186/s40529-018-0224-z