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- Title
Protective Effects of Intestinal Trefoil Factor against Endotoxin-Induced Injury of Intestinal Mucosal Epithelial Cells in Piglets.
- Authors
Zhenyu Chang; Hui Zhang; Mehmood, Khalid; Rehman, Mujeeb Ur; Xiaodan Yuan; Zhaoyang Li; Nabi, Fazul; Xiaoxing Wu; Xinxin Tian; Xueting Liu; Jingen Xu; Donghai Zhou
- Abstract
Individual intestinal epithelial cells can survive and maintain their activity for several hours in tissue samples. For this study piglets without colostrum feeding were selected. Primary cell culture and subculture was performed from the intestinal epithelial cells of piglets. Then the effects of intestinal trefoil factor (TFF3) in intestinal disease was explored. Porcine intestinal epithelial cells were treated with lipopolysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regulated TFF3 mRNA expression. Furthermore, TFF3 mRNA expression was decreased by rhTFF3 treatment. These findings indicate that TFF3 attenuates LPS-induced cell damage, and TFF3 mRNA expression is influenced by LPS and rhTFF3 in intestinal mucous epithelial cells in piglets.
- Subjects
TREFOIL factors; ENDOTOXINS; INTESTINAL mucosa injuries; EPITHELIAL cells; PIGLETS; CELL culture
- Publication
Pakistan Journal of Zoology, 2017, Vol 49, Issue 6, p2249
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2017.49.6.2249.2255