We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Prevalence and molecular characterization of extended-spectrum β–lactamase—producing Escherichia coli isolates from dairy cattle with endometritis in Gansu Province, China.
- Authors
Zhang, Kang; Feng, Haipeng; Zhang, Jingyan; Guo, Zhiting; Yan, Zunxiang; Wang, Guibo; Wang, Xuezhi; Wang, Lei; Li, Jianxi
- Abstract
Background: The present study aimed to investigate the prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL)—producing Escherichia coli (E. coli) isolated from dairy cattle with endometritis in China. The prevalence of ESBL-producing E. coli in sample was detected using ChromID ESBL agar, and genotyping of the ESBL producers was performed by PCR and DNA sequencing. Results: The results revealed that the proportion of positive pathogens tested was 69.76% (180/258) in samples obtained from cows diagnosed with clinical endometritis, with E. coli accounting for 170 out of the 180 positive samples. The infection rate of isolated E. coli was 39.14% (101/258), and co-infections with other pathogens were prevalent. Furthermore, among the 158 E. coli isolates, 50 strains were identified as ESBL producers, with TEM and CTX-M prevalence rates at 78.00% and 32.00%, respectively. Drug sensitivity experiments indicated that 50 isolates of ESBL- producing E. coli were multidrug resistance (MDR), with 48.0% of them exhibiting positive results for both the class 1 integron gene and five gene cassettes associated with resistance to trimethoprim (dfr1 and dfrA17) and aminoglycosides (aadA1, aadA5, and dfrA1), respectively. Conclusion: This investigation demonstrated a substantial prevalence and heightened level of antimicrobial resistance among ESBL-producing E. coli isolates derived from dairy cattle infected with endometritis in China.
- Subjects
CHINA; GANSU Sheng (China); ENTEROBACTERIACEAE; DAIRY cattle; ESCHERICHIA coli; ENDOMETRITIS; MULTIDRUG resistance; DRUG resistance in microorganisms
- Publication
BMC Veterinary Research, 2024, Vol 20, Issue 1, p1
- ISSN
1746-6148
- Publication type
Article
- DOI
10.1186/s12917-023-03868-x