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- Title
Expression of Insulin-Like Growth Factor Binding Protein-3 (IGFBP-3) in the Psoriatic Lesion.
- Authors
Wraight, Christopher J.; Edmondson, Stephanie R.; Fortune, Denys W.; Varigos, George; Werther, George A.
- Abstract
Epidermal hyper-proliferation is a key feature of psoriasis, and a role for IGF-I in this process has previously been proposed. Herein we investigated the expression of IGF binding proteins in the psoriatic lesion and compared it with normal skin. With <em>in situ</em> hybridization, we found that IGFBP-3 mRNA was expressed in the basal layer of the epidermis in nonnal skin. IGFBP-3 was also detected immunohis- tochemically, exclusively in the basal layer. In the psoriatic lesion, IGFBP-3 mRNA was similarly limited to the basal layer despite the characteristic expansion of the basaloid keratinocyte compartment and was detected only in the suprapapillary epidermis, where IGFBP-3 mRNA was more abundant than in normal or uninvolved epidermis, and IGFBP-3 protein could be readily detected with specific antibody. As with IGFBP-3 mRNA, which represents the likely site of IGFBP-3 synthesis, IGFBP-3 was strictly limited to the basal keratinocytes of the suprapapillary epidermis. By using an antibody to the cell cycle antigen Ki67, we also showed that the suprapapillary epidermis, where IGFBP-3 expression was maximal, contained few keratinocytes undergoing mitosis, whereas the tips of the rete pegs, where IGFBP-3 expression was conspicuously absent, contained many keratinocytes undergoing mitosis. We suggest that IGFBP-3 is a growth inhibitor in basal keratinocytes and that absence of IGFBP-3 in the tips of rete pegs may contribute to epidermal hyper-proliferation in the psoriatic lesion.
- Subjects
INSULIN-like growth factor-binding proteins; PSORIASIS; CARRIER proteins; EPIDERMIS; KERATINOCYTES
- Publication
Journal of Investigative Dermatology, 1997, Vol 108, Issue 4, p452
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12289713