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- Title
An easy RHD genotyping strategy for D– East Asian persons applied to Korean blood donors.
- Authors
Luettringhaus, Timo A.; Cho, Duck; Ryang, Dong Wook; Flegel, Willy A.
- Abstract
BACKGROUND: In East Asian populations RHD alleles are known to occur frequently among D– donors, requiring suitable genotyping strategies. The molecular basis of the “ RHD el ” allele previously reported in Taiwan to harbor a genomic 1013-bp deletion was questioned by several authors. STUDY DESIGN AND METHODS: The presence of the RHD gene was investigated in 126 random serologic D– blood donors from Gwangju, southwest Korea. Four donors who typed weakly positive for the D antigen were also analyzed. RH alleles were determined by polymerase chain reaction (PCR) with sequence-specific priming (PCR-SSP) or nucleotide sequencing. RESULTS: Seventy-five percent of the serologically D– samples lacked the RHD gene, 10 percent carried the hybrid RHD-CE(2-9)-D 2 or RHD-CE(2-7)-D 2 alleles, 13 percent represented the RHD(K409K), and 2 percent were weak D type 15 and type 17. Among the four donors typing weak D, two carried weak D type 15, one RHD(K409K), and one the novel weak D type 43. Critical molecular characteristics of RHD(K409K) and its population frequencies were indistinguishable to those reported for the RHD el allele. CONCLUSION: Korean RHD allele frequencies are comparable to Chinese and Japanese frequencies. It is concluded that the RHD el allele may actually not exist but is identical to RHD(K409K). A practical RHD genotyping strategy applicable to D– donors in all East Asian populations was devised. The strategy requires four PCR-SSP procedures only for RHD intron 4 and exon 7 as well as RHD(K409K) and non- RHD(K409K).
- Subjects
ASIA; BLOOD donors; ANTIGENS; POLYMERASE chain reaction; NUCLEOTIDE sequence; INTRONS; EXONS (Genetics)
- Publication
Transfusion, 2006, Vol 46, Issue 12, p2128
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/j.1537-2995.2006.01042.x