We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
1,25-Dihydroxyvitamin D3-Differentiated Human Promyelocytic Leukaemia Cells (HL-60) Can Kill Antibody--Coated Tumour Cells (K562).
- Authors
Espevik, T.
- Abstract
The active metabolite of vitamin D3, 1α,25-dihydrovitamin D3 (1,25(OH)2D3), was tested for the ability to differentiate promyelocytic leukaemia cells (HL-60) and histocytic lymphoma cells (U937) into cytotoxic effector cells against K562 leukaemia cells. A concentration of 10-6 M 1,25(OH)2D3 differentiated HL-60 cells into substrate-adherent monocyte-like cells with cytolytic activity against antibody-coated K562 cells. These differentiated HL-60 cells were not able to lyse uncoated K562 cells in an 18-h Cr-release assay. Similar treatment of the U937 cells with 1,25(OH)2D3, did not make them cytolytic towards K562 cells. These data indicate that 1,25(OH)2D3 can dedifferentiate HL-60 cells to mature monocytes with cytolytic activity against antibody-coated leukaemia cells.
- Subjects
LEUKEMIA; IMMUNOGLOBULINS; LYMPHOMAS; LEUCOCYTES; STEROID hormones; VITAMIN D; ANEMIA
- Publication
Scandinavian Journal of Immunology, 1985, Vol 22, Issue 1, p105
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1985.tb01865.x