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- Title
Lung ICAM-1 and ICAM-2 support spontaneous intravascular effector lymphocyte entrapment but are not required for neutrophil entrapment or emigration inside endotoxin-inflamed lungs.
- Authors
Petrovich, Ekaterina; Feigelson, Sara W.; Stoler-Barak, Liat; Hatzav, Miki; Solomon, Adam; Bar-Shai, Amir; Ilan, Neta; Jin-Ping Li; Engelhardt, Britta; Vlodavsky, Israel; Alon, Ronen
- Abstract
The pulmonary vasculature constitutively expresses the integrin lymphocyte function-associated antigen-1 ligands intercellular adhesion molecule (ICAM)-1 and -2. In this study, effector T cells were temporarily entrapped by the lung vasculature on their way to inflamed lymph nodes, and this entrapment was strongly reduced in ICAM-1 and -2 double-deficient mice (79 and 86% reduction for CD8+ and CD4+ effectors, respectively, compared with wild-type mice). Although the pulmonary vasculature has been suggested to be masked by the heparan sulfate-containing glycocalyx, which is susceptible to heparanase-mediated shedding, lung and lymphocyte heparanase have been found to be unnecessary for this entrapment. Systemic LPS induced rapid neutrophil entrapment in the lung vasculature, but in contrast to T-cell entrapment, this sequestration was ICAM-1, ICAM-2, and heparanase independent. Furthermore, neutrophil migration into the bronchoalveolar space induced by LPS inhalation and LPS-induced leakage of red blood cells into this space were not dependent on lung ICAMs or heparanase activity. Nevertheless, heparanase was critical for neutrophil accumulation in smoke-exposed lungs. Our results indicate that, whereas T cells use ICAM-1 and -2 for temporary pulmonary entrapment, neutrophils get sequestered and extravasate into inflamed lungs independent of ICAMs. This is the first demonstration that the pulmonary vasculature is differentially recognized by T cells and neutrophils.
- Subjects
LYMPHOCYTE transformation; NEUTROPHIL immunology; ENDOTOXIN analysis; PNEUMONIA; LABORATORY mice; LIGANDS (Biochemistry)
- Publication
FASEB Journal, 2016, Vol 30, Issue 5, p1767
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.201500046