We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Potential application of aqueous two-phase systems and three-phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of K luyveromyces marxianus.
- Authors
Simental‐Martínez, Jesús; Rito‐Palomares, Marco; Benavides, Jorge
- Abstract
Superoxide dismutase (SOD; EC 1.15.1.1) is an antioxidant enzyme that represents the primary cellular defense against superoxide radicals and has interesting applications in the medical and cosmetic industries. In the present work, the partition behavior of SOD in aqueous two-phase systems (ATPS) (using a standard solution and a complex extract from Kluyveromyces marxianus as sample) was characterized on different types of ATPS (polymer-polymer, polymer-salt, alcohol-salt, and ionic liquid (IL)-salt). The systems composed of PEG 3350-potassium phosphate, 45% TLL, 0.5 M NaCl (315 U/mg, 87% recovery, and 15.1-fold purification) and t-butanol-20% ammonium sulfate (205.8 U/mg, 80% recovery and 9.8-fold purification), coupled with a subsequent 100 kDa ultrafiltration stage, allowed the design of a prototype process for the recovery and partial purification of the product of interest. The findings reported herein demonstrate the potential of PEG-salt ATPS for the potential recovery of SOD. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:1326-1334, 2014
- Subjects
KLUYVEROMYCES marxianus; SUPEROXIDE dismutase; HYDROPHILIC compounds; POLYMER-polymer complexes; POTASSIUM phosphates
- Publication
Biotechnology Progress, 2014, Vol 30, Issue 6, p1326
- ISSN
8756-7938
- Publication type
Article
- DOI
10.1002/btpr.1979