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- Title
Ameliorating effect of vitamin E on in vitro development of preimplantation buffalo embryos.
- Authors
Thiyagarajan, B.; Valivittan, K.
- Abstract
Purpose Oxidative stress has been implicated in the etiology of defective embryo development. Vitamin E is an effective lipid-soluble antioxidant, protecting cell membranes from peroxidative damage. In this context, this study was undertaken to find if supplementation of vitamin E in culture medium could ameliorate the developmental competence of preimplantation buffalo embryos. Methods Vitamin E was supplemented in maturation/ embryo culture medium at concentrations of 0, 50, 100, 200 and 400 μM. The developmental competence of buffalo embryos was assessed by observing the cleavage, morulae, blastocyst rate, total cell count and comet assay. Results Vitamin E had no significant effect in maturation medium. Vitamin E in embryo culture medium under 5% O2 significantly reduced blastocyst formation in the 400 μM supplemented group. Culture under 20% O2 enhanced the frequency of blastocyst formation, total cell count and significantly reduced comet tail in the 100 μM supplemented group (P<0.001) when compared with the control. Vitamin E in ECM for the first 72 h of culture period enhanced blastocyst rate and total cell number in the 100 μM group (P<0.001) when compared with the control. Conclusion Our results demonstrate that the addition of Vitamin E may enhance the developmental competence of buffalo embryos in vitro by protecting them from oxidative stress.
- Subjects
EMBRYO anatomy; FERTILIZATION in vitro; PREIMPLANTATION genetic diagnosis; VITAMIN E; CELL membranes
- Publication
Journal of Assisted Reproduction & Genetics, 2009, Vol 26, Issue 4, p217
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-009-9302-1