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- Title
Cyclic voltammetric acetylcholinesterase biosensor for the detection of captan in apple samples with the aid of chemometrics.
- Authors
Nesakumar, Noel; Sethuraman, Swaminathan; Krishnan, Uma; Rayappan, John
- Abstract
The presence of captan residues in apples shows high toxicity, which often causes eye and skin irritation, dermatitis, conjunctivitis, and vomiting in humans. In this context, an electrochemical biosensor based on acetylcholinesterase (AChE) immobilized on a ZnO nanorod interface has been proposed. In this work, Hill, dose-response, and first-, second-, and third-order polynomial regression models were successfully applied and the prediction ability of these models was tested with the use of current density obtained from the cyclic voltammograms of appropriate captan solutions. The Pt/ZnO/AChE bioelectrode showed a high sensitivity of 0.538 μA cm μM in the linear range from 0.05 to 25.0 μM with a limit of detection of 107 nM. The recovery results were observed between 98.4 and 102.4 % from the apple sample. This work provides a new promising tool for the detection of captan in apple samples.
- Subjects
CAPTAN; ACETYLCHOLINESTERASE; SKIN inflammation; CONJUNCTIVITIS; ACETYLCHOLINESTERASE inhibitors
- Publication
Analytical & Bioanalytical Chemistry, 2015, Vol 407, Issue 16, p4863
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-015-8687-1