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- Title
Expression of prostaglandin E<sub>2</sub> receptor subtypes, EP2 and EP4, in the rat hippocampus after cerebral ischemia and ischemic tolerance.
- Authors
Jeong-Sun Choi; Ha-Young Kim; Myung-Hoon Chun; Jin-Woong Chung; Mun-Yong Lee
- Abstract
We investigated the distribution and time course of expression of two subtypes of prostaglandin E2 (PGE2) receptors, EP2 and EP4, in a rat model of cerebral ischemia and ischemic tolerance. Adult male Sprague-Dawley rats were subjected to either lethal global ischemia (10 min) with or without sublethal ischemic preconditioning (3 min), or ischemia only (3 min). A short 3-min cerebral ischemia and a 3-min ischemia followed by a second lethal ischemia enhanced the expression of EP2 and EP4 receptors in CA1 pyramidal neurons of the hippocampus. In tolerance-acquired CA1 neurons, the immunoreactivities of EP2 and EP4 were upregulated after 4 h and 12 h, respectively. The immunoreactivities were most prominent at 3 days and were sustained for at least 14 days, consistent with results of immunoblotting experiments. However, immunoreactivities for these PGE2 receptors increased in reactive glial cells in the vulnerable CA1 and hilar regions of rats subjected to lethal ischemia without ischemic preconditioning. Most of the EP2 immunoreactivity occurred in microglial cells and some astrocytes, whereas increased immunoreactivity for EP4 was found only in astrocytes. These data suggest that ischemia and the induction of ischemia tolerance have different regulatory effects on the expression of EP2 and EP4 receptors. Moreover, PGE2 may exert its unique pathophysiological functions in relation to delayed neuronal death and ischemic tolerance induction in the rat hippocampus via specific PGE2 receptors.
- Subjects
PROSTAGLANDINS; CEREBRAL ischemia; ISCHEMIA; ANIMAL models in research; ASTROCYTES; BLOOD circulation disorders
- Publication
Cell & Tissue Research, 2006, Vol 324, Issue 2, p203
- ISSN
0302-766X
- Publication type
Article
- DOI
10.1007/s00441-005-0121-0