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- Title
Clonorchis sinensis: molecular cloning and functional expression of a novel cytosolic glutathione transferase.
- Authors
Xuchu Hu; Jin Xu; Shouyi Chen; Zhongdao Wu; Xinbing Yu
- Abstract
Abstract??Glutathione transferases (GSTs) represent a large family of enzymes. In the high throughput sequencing of the cDNA library constructed from the adult stage ofClonorchis sinensis(Cs), we isolate another cDNA clone encoding a novel cytosolic GST enzyme. To discriminate with our former reported CsGST, we designated this GST as CsGST1. This new cDNA contains 744?bp with a putative open reading frame of 213 amino acids. The deduced amino acid sequence exhibits 88% identity toOpisthorchis viverrini28GST (Ov28GST), 60 and 52% identity toC.sinensiscytosolic 28-kDa GST (Cs28GST) and CsGST, respectively. The CsGST1 was expressed inEscherichia coliBL21(DE3) as a His-tag fusion protein and was purified by Ni-NTA agarose. The recombinant CsGST1 showed moderate GST activity of 0.79?U mg?1. The averageKmof the CsGST1 for 1-chloro-2, 4-dinitrobenzene is 150??M. Cibacron blue F3GA and albendazole inhibited the CsGST1 activity with average IC50values of 9.1 and 265.4??M, respectively. The nucleotide sequence reported in this paper was submitted to the GenBank Database with accession number DQ342327.
- Subjects
MOLECULAR cloning; GLUTATHIONE transferase; TRANSFERASES; ENZYMES
- Publication
Parasitology Research, 2007, Vol 100, Issue 2, p227
- ISSN
0932-0113
- Publication type
Article