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- Title
PORPHYRINS WITH FORMYL GROUPS VI.
- Authors
Lemberg, R.; Stewart, M.
- Abstract
Methods are described for the purification of porphyrin α from heart muscle. After removal of protoporphyrin and a little cryptoporphyrin α by extraction from ether with 8 p.c. HCl, porphyrin α is further purified from tenaciously held lipoids by ehromatography on cellulose powder and on silica gel columns, and by fractional extraction with solvents. This gives porphyrin αα. By treatment with aqueous hydrochloric acid, porphyrin αα is partly converted into porphyrin αβ which has the same absorption spectrum, but differs from porphyrin αα in its distribution between ether and hydrochloric acid, ether and phosphate buffer, and in its chromatographic behaviour. Porphyrins αα and αβare separated by these methods, and both are obtained in the same high degree of purity. Both are stable to alkali. Haem α is reconstituted from porphyrin α by introduction of iron, and the iron removed again without alteration of the spectroscopic properties of porphyrin α. Porphyrin αβ is, however, converted to porphyrin αα during the removal of iron. The problem of whether the existence of the two interconvertible forms is related to the existence of the two cytochromes, α and α3, in heart muscle, must therefore remain open. Crystalline porphyrin α was obtained from reconstituted haemin. The properties of porphyrin α and several of its compounds, including some haematin α compounds, are described. The relevance of these observations to the problem of the structure of porphyrin α, to the properties of cytochrome a and cytochrome oxidase, and to the incorporation of the latter in mitochondria are discussed.
- Subjects
PORPHYRINS; MYOCARDIUM; LIPIDS; IRON; SPECTRUM analysis
- Publication
Australian Journal of Experimental Biology & Medical Science, 1955, Vol 33, Issue 4, p451
- ISSN
0004-945X
- Publication type
Article
- DOI
10.1038/icb.1955.48