We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
PRODUCTION OF AMINOBENZOIC ACIDS BY BACTERIA RESISTANT TO SULPHONAMIDES.
- Authors
Lemberg, R.; Callaghan, J. P.; Tandy, D. E.; Goldsworthy, N. E.
- Abstract
A method for the separation and identification of small amounts of p- and o-aminobenzoic acids has been developed. The acids are diazotized and coupled with dimethyl-a-naphthylamine and the azo dyes so formed are separated by chromatography. A strain of <em>Bact. coli commune</em>, growing in synthetic Kisch's medium, produced diazotizable amino acids in the supernatant. The major part of these could be shown to consist of anthranilic acid with only a small amount of p-aminobenzoic acid. After this strain had become adapted to sulphathiazole, it did not produce more p-aminobenzoic acid than the parent susceptible strain, and the amount of p-aminobenzoic acid was insufficient to account for the resistance to sulphathiazole. The same held for the sum of o- and p-aminobenzoic acid produced. The production of anthranilic acid was variable and decreased with the number of sub-cultures. The characteristic differences between the dye derived from o-aminobenzoic acid and those derived from p- and m-aminobenzoic acids are explained as due to the chelation of the o-carboxylic acid group with the azo group nitrogen by aluminium or hydrogen linkage.
- Subjects
AMINOBENZOIC acids; AZO dyes; AMINO acids; CARBOXYLIC acids; NITROGEN
- Publication
Australian Journal of Experimental Biology & Medical Science, 1948, Vol 26, Issue 1, p9
- ISSN
0004-945X
- Publication type
Article
- DOI
10.1038/icb.1948.2