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- Title
番茄青枯病拮抗菌株 B-6 的诱变选育.
- Authors
李甜爽; 王潞伟; 任潇妍; 刘海霞; 王美琴
- Abstract
Bacterial wilt caused by Ralstonia solanacearum is an important soil-borne disease in the production of protected tomatoes, which can result in huge economic losses for growers. Screening out mutant strains with high control efficiency has become one of the important methods. To provide a basis for the further development and application of biocontrol agents, in this study, Pseudomonas aeruginosa B-6 with antibacterial activity was selected as the starting strain and mutated by the UV-NaNO2 compound. The optimal mutation conditions were determined by the lethality rate and positive mutation rate, and the highefficiency mutant strains were first screened on the plate and re-screened by fermentation in shake flasks. The genetic stability of the antibacterial activity of the high-efficiency mutant strain was determined and the growth curve was compared with that of the starting strain. The results showed that a mutant strain UH-9 with high efficiency and the strongest antibacterial activity was selected after the combined treatment of 3 min of ultraviolet irradiation and 0.045 mol/L of sodium nitrite concentration. The average diameter of the inhibition zone against Ralstonia solanacearum reached up to 28.6 mm, and the activity increased by 86.5% compared with that of the starting strain. After subculture for seven generations, the mutant strain UH-9 was proved to be genetically stable since the antibacterial activity had no significant difference. The antibacterial activity of composite mutagenesis treatment was 39.1% and 25.4% higher than that of single UV mutagenesis treatment and sodium nitrite mutagenesis treatment, respectively. The antibacterial activity of composite mutagenesis was better than that of single mutagenesis. By comparing the growth curves of strain UH-9 and strain B-6, the growth period was the same, and the OD600 of strain UH-9 was higher than that of strain B-6 after 13 h.
- Subjects
BACTERIAL wilt diseases; RALSTONIA solanacearum; SODIUM nitrites; ANTIBACTERIAL agents; BIOLOGICAL pest control agents; PSEUDOMONAS aeruginosa
- Publication
Journal of Shanxi Agricultural Sciences, 2023, Vol 51, Issue 7, p810
- ISSN
1002-2481
- Publication type
Article
- DOI
10.3969/j.issn.1002-2481.2023.07.13