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- Title
ISOLATION SCREENING AND OPTIMIZATION OF LACCASE ENZYME PRODUCING FUNGI FROM MANGROVE SEDIMENT, PARANGIPETTAI.
- Authors
Basha, S. Mjm; Parivalla, M.; Harikrishnan, S.; Rahman, M. Syed Abdul; Sivasubramani, K.; Velammal, A.; Aiyamperuma, B.; Anantharaman, P.
- Abstract
Laccases are vital players in the degradation of lignin, a complex polymer found in plant cell walls. They play a vital role in the ligninolytic activity of white-rot fungi, known for breaking down lignin the present study aimed to isolate a marine fungus with promising laccase-producing potential from the sediment. The present study collected sediment samples from mangrove sediment, Parangipettai area of Tamil Nadu, India. They were used to screen and isolate a promising marine fungus for laccase production. A total of 11 fungi were isolated. Among them, the isolate MSJM 06 showed the highest laccase activity (2.8 cm), The most potential strain, A. fumigatus was identified through LPCB, SEM and Molecular identification using ITS rRNA Sequencing and it was further selected for optimization and laccase production. Potential strains of MJMS-06 required 88 hours of incubation, 80 rpm agitation, pH 8, Temperature 37°C, 1.8% salinity, 1.5% starch as the carbon source was maltose and 0.7% peptone as the nitrogen source.
- Subjects
TAMIL Nadu (India); INDIA; LACCASE; PLANT cell walls; ASPERGILLUS fumigatus; MARINE fungi; MANGROVE plants; PLANT polymers
- Publication
Journal of Experimental Zoology India, 2024, Vol 27, Issue 1, p209
- ISSN
0972-0030
- Publication type
Article
- DOI
10.51470/jez.2024.27.1.209