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- Title
Effect of honey use with Seriphidium chitralense podlech on growth and biofilm formation of Pseudomonas aeruginosa.
- Authors
Shabbir, Urooj; Mumtaz, Sameena; Khan, Imran; Yameen, Muhammad Arfat; Riaz, Muhammad; Khan, Uzma; Ilyas, Noshin; Nawaz, Ismat; Ahmad, Rizwan; Khan, Wajiha
- Abstract
The combined use of honey and medicial plants has a history of traditional usage in various ailments. The present work is a scientific evaluation of honey and crude extracts of Seriphidium chitralense Pod (Sc) against Pseudomonas aeruginosa (Pa) biofilms. Biofilms are intricate microbial communities linked with insistent infections showing increased antimicrobial and immunological resistance. The agar well diffusion (AWD) method has been used to determine antimicrobial activities. The broth microdilution method was used to determine the MIC of honey (Manuka and Gilgit honey) and different plant extracts against Pa. Both honey and extracts exhibited antibacterial activity against the test organism, in which Gilgit honey and aqueous extract showed significant antibacterial activity. In the case of AWD, Gilgit honey showed low activity at a concentration of 10%, while Manuka honey showed low activity at 5%. This study also reports the inhibitory effect of honey and medicinal plants on biofilm formation by Pa. The plant extract was prepared in ethyl acetate, methanol, and distilled water. Plant extract in distilled water showed maximum biofilm inhibition compared to methanol and ethyl acetate in M63 medium. MICs of honey with Sc extracts were determined to search for synergistic combinations against Pa, which showed anti-bacterial solid activity compared to honey and plant extract alone. Manuka honey, in combination with aqueous extracts, showed high activity (0.0266), and Gilgit honey, in combination with aqueous extracts, showed significant activity (0.0432) compared to the other Sc extracts. The results of this study revealed that honey-plant extract mixtures have the potential to serve as a cheap source of anti-bacterial agents in pharmaceutical formulations and alternative products.
- Subjects
PSEUDOMONAS aeruginosa; HONEY; BIOFILMS; PLANT extracts; ANTIBACTERIAL agents; ETHYL acetate
- Publication
Kuwait Journal of Science, 2023, Vol 50, Issue 4, p703
- ISSN
2307-4108
- Publication type
Article
- DOI
10.1016/j.kjs.2023.05.017