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- Title
Stereotypic Rheumatoid Factors That Are Frequently Expressed in Mucosa-Associated Lymphoid Tissue-Type Lymphomas Are Rare in the Labial Salivary Glands of Patients With Sjögren's Syndrome.
- Authors
Bende, Richard J.; Slot, Linda M.; Hoogeboom, Robbert; Wormhoudt, Thera A. M.; Adeoye, Akanbi O.; Guikema, Jeroen E. J.; Noesel, Carel J. M.
- Abstract
Objective Among autoimmune diseases, Sjögren's syndrome (SS) is most strongly associated with the development of malignant B cell lymphoma, in particular mucosa-associated lymphoid tissue (MALT)-type lymphoma. Previously, we have shown that in ∼40% of cases of salivary gland MALT lymphoma, high-affinity stereotypic rheumatoid factor (RF) B cell receptors, specific for IgG-Fc, are expressed. This study was undertaken to investigate whether in the inflamed salivary glands of patients with SS, a similar RF-biased Ig repertoire is present. Methods Extensive analyses of the B cell Ig VH region repertoire were performed on microdissected tissue samples from the labial salivary glands of 4 patients with SS. Results All SS labial salivary glands harbored expanded B cell clones, of which 1 or 2 were highly expanded and detected in >50% of the microdissected samples. However, among the identified 464 distinct Ig clonotypes, only 3 stereotypic RF-expressing clones were detected. In 2 patients with SS, an RF-expressing clone was detected at low frequency in 1 of the microdissected samples, whereas 1 patient with SS harbored a highly expanded RF-expressing clone that was detected in all microdissected samples and also detected in the peripheral blood. Two years after analysis of this sample, the latter patient developed a diffuse large B cell lymphoma originating from the same RF clone. Conclusion Inflamed labial salivary glands in patients with SS generally harbor 1 or 2 highly expanded B cell clones. The repertoire strongly biased toward stereotypic RFs in salivary gland MALT lymphomas is not a reflection of a similar repertoire in the inflamed salivary glands of patients with SS; rather, in the latter, the repertoire is based on a strong selection advantage of incidental stereotypic RF-expressing B cells.
- Subjects
NETHERLANDS; ACADEMIC medical centers; AUTOANTIBODIES; B cell lymphoma; ENZYME-linked immunosorbent assay; IMMUNOHISTOCHEMISTRY; POLYMERASE chain reaction; SALIVARY glands; SJOGREN'S syndrome; REVERSE transcriptase polymerase chain reaction
- Publication
Arthritis & Rheumatology, 2015, Vol 67, Issue 4, p1074
- ISSN
2326-5191
- Publication type
Article
- DOI
10.1002/art.39002