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- Title
Identification and Differentiation of Holstein Cattle Umbilical Cord Mesenchymal Stem Cells.
- Authors
ZONG Junlin; WANG Yaqin; LIU Yanchen; XU Long; GUAN Weijun
- Abstract
As important genetic germ plasm resources and new potential cells for regenerative medicine, animal stem cells are broadly researched and utilized. In this study, Holstein bovine umbilical cord mesenchymal stem cells (UCMSCs) were isolated and investigated, and their multi-directional differentiation potential etc. biological characteristics were researched. The cells were isolated from 3-month-old fetal umbilical cords of Holstein cows using type IV collagenase, cultured in vitro. The obtained cells showed a typical "S" shape growth curve, and the population doubling time gradually decreased with the increase of generation. The expression of specific surface marker genes in isolated cells were identified by immunofluorescence and RT-PCR techniques, the results showed that CD29, CD73, CD90 and CD166 expressed, while CD45 didn' t express. After induction of adipogenesis, lipid droplets stained with oil red stain were seen, and adipogenic genes LPL and PPAR-γ both expressed. After induction of osteogenic differentiation, calcium nodules were stained with alizarin red, and osteogenic genes OPN and collagen type I gene COL-I expressed; after induction of chondrogenic differentiation, the chondrocytes were stained with Alisin blue, and they expressed chondrogenic key gene transcription factor gene SOX9 and ACAN. In this study, mesenchymal stem cells with good proliferation viability and adipogenic, osteogenic and chondrogenic abilities were successfully isolated from the umbilical cord of Holstein cattle at 3-month-old embryonic, which provided basis for the conservation of animal germplasm resources and regenerative medicine as well as potential cell resources.
- Subjects
ADIPOGENESIS; HOLSTEIN-Friesian cattle; MESENCHYMAL stem cells; UMBILICAL cord; GERMPLASM; GERMPLASM conservation
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2023, Vol 25, Issue 9, p105
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2023.0401