We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phospholipid Composition of Human Blood Plasma as Detected by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: New Observations.
- Authors
Milman, B. L.; Lugovkina, N. V.; Zhurkovich, I. K.
- Abstract
The dependence of matrix-assisted laser desorption/ionization (MALDI) mass spectra of lipids extracted from human blood plasma and corresponding lipid compositions on different experimental conditions was studied. Not only numerous compounds of the lysophosphatidylcholines (LPCs) and phosphatidylcholines (PCs) groups, but also lipid oxidation products, including six oxidized LPCs, are detected. They are present in the original samples and produced by the reaction with air oxygen molecules, resulted in their accumulation in samples under analysis. A higher sensitivity of MALDI detection is observed for less hydrophobic LPCs as compared to more hydrophobic PCs; this phenomenon is explained. The hydrophobicity/lipophilicity factor is also revealed in replacing the matrix of 2,5-dihydroxybenzoic acid by α-cyano-4-hydroxycinnamic acid. The latter matrix is rarely used in MALDI detection of lipids and may be suitable for the selective determination of LPCs and oxidized LPCs.
- Subjects
PHOSPHOLIPIDS; BLOOD plasma; ELECTROSPRAY ionization mass spectrometry; MATRIX-assisted laser desorption-ionization; LYSOPHOSPHATIDYLCHOLINE acyltransferase
- Publication
Journal of Analytical Chemistry, 2017, Vol 72, Issue 14, p1411
- ISSN
1061-9348
- Publication type
Article
- DOI
10.1134/S1061934817140088