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- Title
Expression of glycine receptor subunits and gephyrin in single bipolar cells of the rat retina.
- Authors
Enz, Ralf; Bormann, Joachim
- Abstract
We studied the expression of glycine receptor (GlyR) subunits and gephyrin in the adult rat retina. Reverse transcribed RNA was amplified by polymerase chain reaction (RT-PCR) with primers designed to recognize GlyR α1, α2, α3, β subunits, and gephyrin. Using RNA isolated from the whole retina, signals for all four GlyR subunits and gephyrin could be observed. In rod bipolar cells, in contrast, we detected a subset of GlyR subunits, α1 and β, and no gephyrin. Patch-clamp recording employing two subtype-specific blockers of the GlyR, picrotoxinin and cyanotriphenylborate (CTB), indicated that the GlyR in rod bipolar cells is a heteromeric protein composed of the α1 and β subunit. Moreover, the absence of detectable amounts of gephyrin mRNA suggests that the anchor protein is not required for the function of GlyRs in rod bipolar cells.
- Publication
Visual Neuroscience, 1995, Vol 12, Issue 3, p501
- ISSN
0952-5238
- Publication type
Article
- DOI
10.1017/S0952523800008403