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- Title
Vitamin D3 mediates miR-15a-5p inhibition of liver cancer cell proliferation via targeting E2F3.
- Authors
Li, Yulong; Lin, Qiang; Chang, Su'E; Zhang, Rong; Wang, Jingjie
- Abstract
Vitamin D3 has been demonstrated to suppress the development and progression of liver cancer, but the mechanism is unclear. The effects of vitamin D3 and microRNA (miR)-15a-5p on liver cancer cells were investigated in the present study using MTT and colony formation assays, flow cytometry, western blotting and reverse transcription-quantitative PCR. A dual-luciferase reporter assay was performed to determine whether E2F transcription factor 3 (E2F3) was a target of miR-15a-5p. The effects of silencing the E2F3 gene expression in liver cancer cells were investigated using a small interfering RNA. Vitamin D3 suppressed liver cancer cell proliferation, induced apoptosis and increased miR-15a-5p expression. Treatment with the miR-15a-5p mimics significantly suppressed liver cancer cell proliferation compared with that of the controls. Bioinformatics analysis and a dual-luciferase reporter assay demonstrated that E2F3 was a target of miR-15a-5p and that silencing E2F3 inhibited liver cancer cell proliferation. Therefore, Vitamin D3 suppressed cell proliferation by miR-15a-5p-mediated silencing of E2F3 gene expression. These findings suggested a role for vitamin D3 and E2F3 targeting as potential novel liver cancer therapies.
- Subjects
CANCER cell proliferation; CHOLECALCIFEROL; LIVER cancer; LIVER cells; GENE silencing
- Publication
Oncology Letters, 2020, Vol 20, Issue 1, p292
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2020.11572