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- Title
The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching.
- Authors
Benetti, F.; Briso, A. L. F.; Carminatti, M.; Araújo Lopes, J. M.; Barbosa, J. G.; Ervolino, E.; Gomes‐Filho, J. E.; Cintra, L. T. A.
- Abstract
Aim: To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology: The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t‐test and Wilcoxon signed‐rank test were used (P < 0.05). Results: The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS‐positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05). Conclusions: Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti‐ROS was involved in cellular defence against H2O2.
- Subjects
OSTEOCALCIN; OSTEOPONTIN; REACTIVE oxygen species; DENTAL pulp; TOOTH whitening; DENTAL discoloration; MOLAR abnormalities; HYDROGEN peroxide
- Publication
International Endodontic Journal, 2019, Vol 52, Issue 5, p665
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/iej.13049