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- Title
A Novel Antimicrobial Endolysin, LysPA26, against Pseudomonas aeruginosa.
- Authors
Mingquan Guo; Chunyan Feng; Jie Ren; Xuran Zhuang; Yan Zhang; Yongzhang Zhu; Dong, Ke; He, Ping; Xiaokui Guo; Jinhong Qin
- Abstract
The global increase in multidrug resistant (MDR) bacteria has led to phage therapy being refocused upon. A novel endolysin, LysPA26, containing a lysozyme-like domain, was screened against Pseudomonas aeruginosa in this study. It had activity against MDR P. aeruginosa without pretreatment with an outer-membrane permeabilizer. LysPA26 could kill up to 4 log units P. aeruginosa in 30 min. In addition, temperature and pH effect assays revealed that LysPA26 had good stability over a broad range of pH and temperatures. Moreover, LysPA26 could kill other Gram-negative bacteria, such as Klebsiella pneumonia, Acinetobacter baumannii and Escherichia coli, but not Gram-positive bacteria. Furthermore, LysPA26 could eliminate P. aeruginosa in biofilm formation. Our current results show that LysPA26 is a new and promising antimicrobial agent for the combat of Gram-negative pathogens.
- Subjects
PSEUDOMONAS aeruginosa; MULTIDRUG resistance in bacteria; PEPTIDOGLYCAN hydrolase
- Publication
Frontiers in Microbiology, 2017, Vol 8, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2017.00293