We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Molecular diagnostics of some trichodorid nematodes and associated Tobacco rattle virus.
- Authors
Boutsika, K.; Phillips, M. S.; MacFarlane, S. A.; Brown, D. J. F.; Holeva, R. C.; Blok, V. C.
- Abstract
Several Paratrichodorus and Trichodorus (trichodorid) nematode species are natural vectors of Tobacco rattle virus (TRV) and cause economically important diseases, especially in potato and ornamental bulbous crops. Identification of trichodorid species based on morphological characters is laborious, time-consuming, and requires the services of highly trained personnel. Molecular diagnostics for trichodorid nematodes, using the ribosomal DNA repeat unit, were successfully developed to distinguish two Paratrichodorus and two Trichodorus species. The complete sequences of the 18S genes and the ITS-1 regions for these species were obtained and species-specific primers successfully designed for them. An RT-PCR assay was developed utilizing isolate-specific primers that amplify serologically distinguishable strains of TRV in individual trichodorid nematodes. The primers were based on the highly conserved RNA-1 segment of the bipartite genome and also on different parts of the RNA-2 segment of the virus genome.
- Subjects
TRICHODORUS; TRICHODORIDAE; TOBACCO rattle virus; DIAGNOSTIC use of polymerase chain reaction; MOLECULAR diagnosis; MOLECULAR biology
- Publication
Plant Pathology, 2004, Vol 53, Issue 1, p110
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1046/j.1365-3059.2003.00938.x