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- Title
Robust single-cell DNA methylome profiling with snmC-seq2.
- Authors
Luo, Chongyuan; Rivkin, Angeline; Zhou, Jingtian; Sandoval, Justin P.; Kurihara, Laurie; Lucero, Jacinta; Castanon, Rosa; Nery, Joseph R.; Pinto-Duarte, António; Bui, Brian; Fitzpatrick, Conor; O’Connor, Carolyn; Ruga, Seth; Van Eden, Marc E.; Davis, David A.; Mash, Deborah C.; Behrens, M. Margarita; Ecker, Joseph R.
- Abstract
Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies.
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-06355-2