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- Title
A Genetic Screen for Saccharomyces cerevisiae Mutants That Fail to Enter Quiescence.
- Authors
Lihong Li; Miles, Shawna; Breeden, Linda L.
- Abstract
Budding yeast begin the transition to quiescence by prolonging G1 and accumulating limited nutrients. They undergo asymmetric cell divisions, slow cellular expansion, acquire significant stress tolerance and construct elaborate cell walls. These morphologic changes give rise to quiescent (Q) cells, which can be distinguished from three other cell types in a stationary phase culture by flow cytometry. We have used flow cytometry to screen for genes that are required to obtain the quiescent cell fraction. We find that cell wall integrity is critical and these genes may help define quiescence-specific features of the cell wall. Genes required to evade the host innate immune response are common. Thesemay be new targets for antifungal drugs. Acquired thermotolerance is also a common property, and we show that the stress-response transcription factors Msn2 and Msn4 promote quiescence. Many other pathways also contribute, including a subset of genes involved in autophagy, ubiquitin-mediated proteolysis, DNA replication, bud site selection, and cytokinesis.
- Subjects
SACCHAROMYCES cerevisiae; FUNGAL cell walls; GENES
- Publication
G3: Genes | Genomes | Genetics, 2015, Vol 5, Issue 8, p1783
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1534/g3.115.019091