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- Title
Establecimiento in vitro de dos especies nativas de Costa Rica: Terminalia amazonia (Amarillón) y Vochysia allenii (Botarrama Blanco).
- Authors
Hine Gómez, Ana; Rojas Vargas, Alejandra; Daquinta Gradaille, Marcos
- Abstract
The objective of this research was to achieve the in vitro establishment of the species Terminalia amazonia and Vochysia allenii due to the difficulty propagate sexually and asexually with conventional techniques. It was possible to establish the nodal segments of both species in in vitro conditions using 0.1 % HgCl2 with an exposure time of 5 minutes. The best nodal culture medium was 100 % WPM salts in T.amazonia and V.allenii was WPM 50% salts. After 28 days of culture 42 % of nodal segments to T.amazonia and V.allenii 10% was obtained. In both species, the effect on sprouting of five concentrations of 6-benzylaminopurine (6-BAP) (0.0, 2.22, 4.44, 6.66, 8.88 μM L-1) and five thidiazuron (TDZ) (0.0, 0.22, 0.45, 0.68, 0.90 μM L-1) was evaluated. Outbreak was scored on average per explant in the five treatments of BAP and TDZ used.
- Subjects
PLANT micropropagation; TERMINALIA; VOCHYSIACEAE; NODES (Botany); PLANT growth promoting substances; THIDIAZURON; BENZYLAMINOPURINE; GERMINATION
- Publication
Revista Colombiana de Biotecnología, 2014, Vol 16, Issue 2, p180
- ISSN
0123-3475
- Publication type
Article