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- Title
Development of nonalcoholic fatty liver disease model by high-fat diet in rats.
- Authors
Wardani, Hijrawati Ayu; Rahmadi, Mahardian; Ardianto, Chrismawan; Balan, Santhra Segaran; Kamaruddin, Norshafarina Shari; Khotib, Junaidi
- Abstract
Background: Nonalcoholic fatty liver disease (NAFLD) is indicated by liver steatosis without excessive alcohol use or other liver disease. Several studies have reported that metabolic syndromes such as obesity, type 2 diabetes mellitus, and dyslipidemia have a linear correlation associated with NAFLD pathophysiology. One of the characteristics of dyslipidemia in NAFLD is increase in serum triglycerides. This study aimed to develop a model of NAFLD characterized by an increase in serum triglyceride levels and histological profile of liver steatosis by high-fat diet in rats. Methods: Twelve Wistar rats were fed with pellets enriched with 60% fat. They were housed individually, and the remaining pellets were weighted every day for intake evaluation. Blood samples were collected at day 0 and at the end of each trial period at days 7, 14, 21, and 28 for the measurement of triglyceride levels. Every animal from each group was also sacrificed for liver histopathological examination. Results: This study has established developing the NAFLD animal model by induction of a high-fat diet. The levels of serum triglycerides were increased from baseline 80.41 ± 12.82 to 1152.00 ± 73.62, 493.66 ± 159.98, 556.00 ± 120.79, and 489.00 ± 156.75 mg/dL at days 7, 14, 21, and 28, respectively. Liver histology also showed liver steatosis development, inflammation, and hepatocellular ballooning, which were associated with the NAFLD state. Conclusions: High-fat diet in rats induced hypertriglyceridemia along with NAFLD-like liver histopathology.
- Subjects
LIVER histology; ANIMAL experimentation; BIOLOGICAL models; FATTY liver; FAT content of food; HYPERLIPIDEMIA; INFLAMMATION; RATS; TRIGLYCERIDES; DESCRIPTIVE statistics; DISEASE risk factors
- Publication
Journal of Basic & Clinical Physiology & Pharmacology, 2019, Vol 30, Issue 6, pN.PAG
- ISSN
0792-6855
- Publication type
Article
- DOI
10.1515/jbcpp-2019-0258