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- Title
Tunicate Endostyles: Histochemistry of Acidic Glycoproteins Re-examined in <em> Ciona intestinalis</em> and <em>Styela plicata</em>.
- Authors
Hohenleitner, Frank J.; Caso, Louis V.
- Abstract
Previous reports of tunicate endostyles have suggested that they contain little or no acidic glycoproteins in the glandular zones The endostyles of Ciona intestinalis and Styela plicata were examined after anhydrous fixation with cyanuric chloride PoIyanions were stained with alcian blue (AB) at pH 2.5 or azure A while sulfomucins were stained with high-iron diamine (HID) or AB at pH 1.0. Endostyles were also tested for sensitivity to acid hydrolysis (AU) and saponification. In Ciona zones 2 and 4 sometimes demonstrated positive HID and AB 10 responses. Almost invariably zone 6 was AB+ at pH 2. 5 zones 2 and 4 acre frequently responsive to AB hut less intense Each ot these 3 zones when AB+ was sensitive to AU Responses by zones 3 and 5 to AB (pH 2.5) azure A and saponification suggest that these zones contain mostly nuclear material In secretory zones 2.4 and 6 histological responses are consistent with the histochemistry of sialomucins Zones 1 and 8 had sulfated material in the apical edges in both animal groups. Among the fixatives used for only anhydrous fixation demonstrated most of the positive responses to polyanion-sensitive stains
- Subjects
TUNICATA; HISTOCHEMISTRY; GLYCOPROTEINS; CIONA intestinalis; CIONA; ANIMAL morphology
- Publication
Acta Zoologica, 1987, Vol 68, Issue 3, p179
- ISSN
0001-7272
- Publication type
Article
- DOI
10.1111/j.1463-6395.1987.tb00889.x