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- Title
Astrocytic Responses to DNA Delivery Using Nucleofection.
- Authors
Muyderman, H.; Yew, W.; Homkajorn, B.; Sims, N.
- Abstract
Nucleofection is a powerful non-viral transfection technique that can deliver plasmid DNA with high efficiency to cells that are traditionally difficult to transfect. In this study, we demonstrate that nucleofection of astrocytes grown in primary cell culture resulted in 76 ± 9% transfected cells and low cytotoxicity. However, the nucleofected astrocytes showed a reduced re-attachment to the growth media when replated and subsequent impairment of proliferation. This led to substantially decreased cell densities during the initial 72 h following transfection. Furthermore, these cells were less efficient at producing wound closure in a scratch model of injury. Nucleofection also resulted in the generation of a small proportion of polynucleated cells. The findings demonstrate that nucleofection provides a valuable technique for delivering DNA to astrocytes in culture. However, considerable care is needed in designing and interpreting such studies because of long-lasting changes induced in key properties of these cells by the nucleofection process.
- Subjects
ASTROCYTES; CELL-mediated cytotoxicity; GENE transfection; DNA; PLASMIDS; CELL culture; CELL proliferation
- Publication
Neurochemical Research, 2010, Vol 35, Issue 11, p1771
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-010-0243-y