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- Title
Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection.
- Authors
Mohamed, Fatma Elzahraa; F. H., Omnia; Deif, Heba N.; Ragab, Eman; El-Jakee, J.
- Abstract
In this work 68 S. aureus isolated from mastitic cows (n=48), camel milk (n=5) and human samples (urine n = 4, abscesses aspiration n = 3, sputum n = 2, nasal swabs n = 2, vaginal swabs n = 1 and wound swabs n = 3) were investigated using phenotypic and molecular identification to detect the occurrence of enterotoxins. Eighteen out of 48 S. aureus isolated from mastitic cows and 5 out of 15 S. aureus isolated from human samples were enterotoxin producers. All toxigenic S. aureus isolated from clinical mastitic cows (13) were toxin type D producer while seb and sed were detected from subclinical mastitic cows. Among human isolates, 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C and D and one isolate produced type C toxin. No toxin was detected from camel isolates. All toxigenic S. aureus isolated from cows (13) were toxin type D producer. Among human isolates 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C & D and one isolate produced type C toxin. Five S. aureus crude enterotoxin type D were investigated by protein SDS-PAGE. It was clear that all isolates had from 9 to 7 bands. All the isolates had a band at 148-159 kDa, 35-42 kDa, 28-30 kDa, 23-26 kDa, 20-22 kDa and 18-19 kDa. 4 isolates had a band at 48-55 kDa, 3 isolates had a band at 39-42 kDa, 3 isolates had a band at 31-34 kDa and one isolate had a band at 17 kDa.
- Subjects
ENTEROTOXINS; STAPHYLOCOCCUS aureus; CAMEL milk; TOXINS; COWS
- Publication
Veterinary Medical Journal, 2020, Vol 66, Issue 1, p98
- ISSN
1110-1423
- Publication type
Article