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- Title
Metal-organic framework-based nanoplatform enhance fibroblast activity to treat periodontitis.
- Authors
Jian JIAO; Yujuan TIAN; Ying LI; Yunkai LIANG; Shu DENG; Wanmeng WANG; Yuwei WANG; Yi LIN; Yuan TIAN; Changyi LI
- Abstract
After periodontal tissue injury, reconstruct soft tissue sealing around the tooth surface is of fundamental importance to treat periodontitis. Among multiple cell types, fibroblast plays a central role in reestablishing functional periodontium. To enhance fibroblast activity, a novel metal-organic framework-based nanoplatform is fabricated using mesoporous Prussian blue (MPB) nanoparticles to load baicalein (BA), named MPB-BA. Drug release test displayed sustained BA release of MPB-BA. Cell proliferation, transwell migration and wound healing tests revealed accelerated fibroblast proliferation and migration for the established MPBBA nanoplatform. Moreover, vinculin immunofluorescence staining, western blot and quantitative real-time PCR analysis showed up-regulated vinculin protein and integrin α5 and integrin β1 gene expressions for MPB-BA, suggesting improved cell adhesion. In addition, hematoxylin and eosin (H&E) and Masson trichromatic staining suggested superior anti-inflammatory and collagen fiber reconstruction effects for MPB-BA in a rat experimental periodontitis model in vivo. Our study may provide a promising strategy for the treatment of periodontitis.
- Subjects
PERIODONTITIS; FIBROBLASTS; PRUSSIAN blue; CELL adhesion; HEMATOXYLIN &; eosin staining; GRAPE seed extract
- Publication
Dental Materials Journal, 2023, Vol 42, Issue 1, p19
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2022-096