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- Title
miR-4458 directly targets IGF1R to inhibit cell proliferation and promote apoptosis in hemangioma.
- Authors
Wu, Maosong; Tang, Yongsheng; Hu, Gang; Yang, Chunjian; Ye, Kaichuang; Liu, Xianluo
- Abstract
Hemangiomas (HAs) are benign neoplasms of the vasculature. MicroRNA-4458 (miR-4458) has been reported to function as a tumor suppressor in multiple malignancies, but its biological function in HAs remains unknown. In the present study, the potential role of miR-4458 in HA-derived endothelial cells (HDECs) was investigated. Firstly, reverse-transcription-quantitative PCR analysis was used to confirm the expression of miR-4458 in HDECs following transfection with miR-4458 mimics or inhibitor. Subsequently, MTT and EdU assays were performed and subsequently determined that miR-4458 overexpression significantly inhibited proliferation, and knockdown promoted cell proliferation in HDECs. Flow cytometry analysis revealed that miR-4458 overexpression induced cell cycle arrest, whereas knockdown reversed G0/G1 phase arrest and apoptosis. Furthermore, insulin-like growth factor 1 receptor (IGF1R) was identified as a target of miR-4458. IGF1R knockdown enhanced the effects of miR-4458 on cell proliferation, cell cycle G0/G1 phase arrest and apoptosis in HDECs. Taken together, the results revealed that miR-4458 targeting of IGF1R may serve as a novel therapeutic strategy for treating patients with HAs.
- Subjects
INSULIN-like growth factor receptors; SOMATOMEDIN C; CELL proliferation; CELL cycle; BENIGN tumors
- Publication
Experimental & Therapeutic Medicine, 2020, Vol 19, Issue 4, p3017
- ISSN
1792-0981
- Publication type
Article
- DOI
10.3892/etm.2020.8546